PERBANDINGAN EFEK PENINGKATAN HDL EKSTRAK BUAH BIT DENGAN EKSTRAK AMPAS TEBU HITAM PADA TIKUS (GALUR WISTAR) YANG DIINDUKSI DIET TINGGI LEMAK
DOI:
https://doi.org/10.5281/zenodo.24001093Keywords:
Black sugarcane bagasse, beetroot, dyslipidemia, extract, flavonoids, HDL, terpenoidsAbstract
Dyslipidemia is a lipid metabolism disorder characterized by elevated plasma lipid levels and is one of the major risk factors for cardiovascular disease (CVD). Simvastatin, a commonly prescribed statin for the management of dyslipidemia, is associated with adverse effects on muscles and the skeletal system. Beetroot (Beta vulgaris L.) and black sugarcane bagasse (Saccharum officinarum L.) extracts contain flavonoids and terpenoids, which are known to have cholesterol-lowering properties. This study aimed to compare the effects of beetroot extract and black sugarcane bagasse extract on increasing high-density lipoprotein (HDL) levels in Wistar rats fed a high-fat diet. The study employed a post-test-only control group design involving 24 male Wistar rats divided into six treatment groups for one month. Total cholesterol levels were analyzed using one-way analysis of variance (ANOVA), followed by the least significant difference (LSD) post hoc test. Following treatment, the mean HDL levels were 33.5 in the normal group receiving a standard diet, 32.5 in the negative control group (K−) receiving quail egg yolk, 37.25 in P1 (beetroot extract at 500 mg/kg body weight), 38.25 in P2 (black sugarcane bagasse extract at 250 mg/kg body weight), 35.75 in P3 (black sugarcane bagasse extract at 500 mg/kg body weight), and 38.5 in the positive control group (K+) receiving simvastatin at 10 mg/kg body weight. The ANOVA results revealed a statistically significant difference among the groups (p = 0.023). Post hoc analysis showed no statistically significant differences between the normal group and P1 or P3; K− and P3; P1 and P2, P3, or K+; P2 and P3 or K+; and P3 and K+. However, statistically significant differences were observed between the normal group and P2 or K+, as well as between K− and P1, P2, or K+. In conclusion, beetroot extract and black sugarcane bagasse extract at doses of 250 mg/kg and 500 mg/kg body weight demonstrated comparable effectiveness in increasing HDL levels in Wistar rats.
References
Akerina, A. (2022). Analisis kandungan kolesterol telur burung puyuh (Coturnix coturnix japonica) yang diberikan pakan komersial dengan penambahan tepung rumput laut. Agrinimal: Jurnal Ilmu Ternak dan Tanaman, 9(2), 92–100. https://doi.org/10.30598/ajitt.2021.9.2.92-100
Al-Dosari, M., Alqasoumi, S., Ahmad, M., Al-Yahya, M., Nazam Ansari, M., & Rafatullah, S. (2011). Effect of Beta vulgaris L. on cholesterol rich diet-induced hypercholesterolemia in rats. Farmacia, 59(5), 669–678.
Fitriani, D., Rusmini, H., & Marek, Y. W. (2019). Pengaruh pemberian ekstrak bunga pepaya (Carica papaya L.) terhadap kadar high density lipoprotein (HDL) dan low density lipoprotein (LDL) darah tikus (Rattus norvegicus) galur Sprague Dawley jantan yang diberi diet tinggi lemak. Jurnal Ilmu Kedokteran dan Kesehatan, 6(4), 247–256. https://doi.org/10.33024/jikk.v6i4.2293
Hidayati, N., & Suryani, S. (2018). Pengaruh pemberian ekstrak air tebu hitam (Saccharum officinarum L.) terhadap kadar kolesterol total serum mencit (Mus musculus) yang diinduksi diet tinggi kolesterol. Biomedika, 2(2), 129–137.
Hussain, A., Kaler, J., & Ray, S. D. (2023). The benefits outweigh the risks of treating hypercholesterolemia: The statin dilemma. Cureus, 15(1), 1–16. https://doi.org/10.7759/cureus.33648
Kim, J. Y., Kim, S. M., Kim, S. J., Lee, E. Y., Kim, J. R., & Cho, K. H. (2017). Consumption of policosanol enhances HDL functionality via CETP inhibition and reduces blood pressure and visceral fat in young and middle-aged subjects. International Journal of Molecular Medicine, 39, 889–899.
Maimunah, S., Amila, A., Marpaung, J. K., Girsang, V. I., & Syapitri, H. (2021). Karakterisasi dan skrining fitokimia dari tepung buah bit (Beta vulgaris L.). Forte Journal, 1(2), 139–145. https://doi.org/10.51771/fj.v1i2.141
Mamat. (2010). Faktor-faktor yang berhubungan dengan kadar kolesterol HDL di Indonesia [Tesis, Universitas Indonesia].
Margata, L., Haloho, D. S. B., & Parhan, P. (2020). Perbandingan efektivitas ekstrak dan jus buah bit (Beta vulgaris L.) terhadap penurunan kolesterol tikus putih. Jurnal Penelitian Farmasi & Herbal, 3(1), 91–99. https://doi.org/10.36656/jpfh.v3i1.330
Maryusman, T., Imtihanah, S., & Firdausa, N. I. (2020). Kombinasi diet tinggi serat dan senam aerobik terhadap profil lipid darah pada pasien dislipidemia. Gizi Indonesia, 43(2), 67–76. https://doi.org/10.36457/gizindo.v43i2.354
Pereira, J. O., Oliveira, D., Faustino, M., et al. (2024). Use of various sugarcane byproducts to produce lipid extracts with bioactive properties: Physicochemical and biological characterization. Biomolecules, 14(2). https://doi.org/10.3390/biom14020233
Rogers, R. (2020). Statins. Chest, 145(1), 13. https://doi.org/10.1378/chest.145.1.13
Shelemo, A. A. (2023). Pengaruh berbagai durasi pemberian diet tinggi lemak terhadap profil lipid tikus putih (Rattus norvegicus strain Wistar) jantan. Nucl Phys, 13(1), 104–116.
Syafitri, A. D., & Mulyani, N. S. (2023). Effect of black sugar cane juice on blood cholesterol levels among patients with hypercholesterolemia. Poltekita: Jurnal Ilmu Kesehatan, 17(1), 8–13.
Teixeira, F. S., Pimentel, L. L., Vidigal, S. S. M. P., Costa, P. T., Pintado, M. E., & Rodriguez-Alcala, L. M. (2022). Lipophilic phytochemicals in sugarcane straw and bagasse. Foods, 11, Article 2661. https://doi.org/10.3390/foods11172661
Wani, A. K., Rahayu, F., Fauziah, L., & Suhara, C. (2023). Advances in safe processing of sugarcane and bagasse for the generation of biofuels and bioactive compounds. Journal of Agriculture and Food Research, 12, Article 100549. https://doi.org/10.1016/j.jafr.2023.100549
